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KMID : 0981820090290030249
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Korean Journal of Laboratory Medicine
2009 Volume.29 No. 3 p.249 ~ p.255
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Comparative Quantification of Plasma hnRNP B1 mRNA in Non-small Cell Lung Cancer Patients by Real-time PCR
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Kim Jung-Man
Hwang Sang-Hyun
Song Eun-Joo
Lee Sang-Yull
Kim Yeong-Dae
Lee Chang-Hun
Lee Min-Ki
Chang Chul-Hun L.
Lee Eun-Yup
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Abstract
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Background: Circulating cell-free nucleic acids are known to be a noninvasive diagnostic tool for cancer detection. Heterogeneous nuclear ribonucleoprotein (hnRNP) B1, a nuclear core complex, is overexpressed in early stage lung cancer. We intended to evaluate the usefulness of plasma hn-RNP B1 mRNA in differentiating non-small cell lung cancer (NSCLC) from other benign lung diseases,especially pulmonary tuberculosis, which is highly prevalent in Korea and often difficult to distinguish from lung cancer.
Methods: Plasma RNA was extracted from 30 patients with NSCLC, 30 patients with benign lung diseases including pulmonary tuberculosis, and 10 healthy controls. Plasma hnRNP B1 mRNA was measured by TaqMan Gene Expression Assay (Applied Biosystems, USA), and pre-developed betaactin (ACTB) mRNA was used for normalization. We analyzed the relative gene expression data using the delta-delta Ct method.
Results: Plasma hnRPN B1 mRNA was measurable in 93.3% (28/30) of NSCLC patients. Normalized 2- Ct of plasma hnRPN B1 mRNA was 62.2 (95%Cl, 6.4-210.1) in NSCLC patients and 2.7 (95% Cl, 0.5-13.6) in benign lung disease patients (P<0.001, Mann-Whitney U test).
Conclusion: Plasma hnRNP B1 mRNA was significantly increased in patients with lung cancer compared with that in patients with other benign lung diseases. Plasma hnRNP B1 mRNA may be useful as a potential marker for the detection of NSCLC.
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KEYWORD
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Lung cancer, hnRNP B1, Plasma mRNA, Cancer biomarker, Circulating nucleic acid
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